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EphA1 antibody

Anti-EphA1 antibody used in Western Blot (WB). GTX57738
Anti-EphA1 antibody used in Flow cytometry (FACS). GTX57738
Anti-EphA1 antibody used in Live cell imaging (LCI). GTX57738
Anti-EphA1 antibody used in Western Blot (WB). GTX57738

Cat. No. GTX57738

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, LCI

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all EphA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)KKEPRQLELTWAGSR, corresponding to amino acid residues 457-471 of human EphA1 (Accession P21709). Extracellular, N-terminus.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EPH , EPH receptor A1 , EPHA1 , EPHT , EPHT1 , EphA1 , Eph receptor A1

Cellular Localization

Cell membrane

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EphA1 antibody used in Western Blot (WB). GTX57738

GTX57738 WB Image

WB analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membrane lysates using GTX57738 EphA1 antibody.
Lane 1 and 3 : Primary antibody
Lane 2 and 4 : Primary antibody preincubated with the control peptide antigen
Dilution : 1:200

Anti-EphA1 antibody used in Flow cytometry (FACS). GTX57738

GTX57738 FACS Image

FACS analysis of Jurkat cells using GTX57738 EphA1 antibody.
Black : Unstained cells
Green : Cells with primary antibody
Dilution : 1:20

Anti-EphA1 antibody used in Live cell imaging (LCI). GTX57738

GTX57738 LCI Image

Live cell imaging analysis of MCF-7 cells using GTX57738 EphA1 antibody.
Panel A : Primary antibody
Panel B : Live view of the cells
Panel C : Merged of panel A and B
Dilution : 1:25

Anti-EphA1 antibody used in Western Blot (WB). GTX57738

GTX57738 WB Image

WB analysis of Jurkat (Lanes 1 and 5), HeLa (Lanes 2 and 6), SH-SY5Y (Lanes 3 and 7), and MCF-7 (Lanes 4 and 8) cell lysates using GTX57738 EphA1 antibody.
Lane 1-4 : Primary antibody
Lane 5-8 : Primary antibody preincubated with the control peptide antigen
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639