APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on internal testing result, this antibody recognizes the extracellular domain of Human EPHA2.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT66G9 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EPHA4 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
EPH receptor A2 , ARCC2 , CTPA , CTPP1 , CTRCT6 , ECK , EPHA2 , EphA2 , Eph receptor A2
Cellular Localization
Cell membrane,Cell projection,Ruffle membrane,Lamellipodium membrane,Cell junction,Focal adhesion
Background
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]
Database
Research Area
DATA IMAGES
![Anti-EphA2 antibody [AT66G9] used in Western Blot (WB). GTX57687](/templates/default/js/lightbox/images/loading.gif "Anti-EphA2 antibody [AT66G9] used in Western Blot (WB). GTX57687")
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GTX57687 WB Image
WB analysis of various samples using GTX57687 EphA2 antibody.
Lane 1 : Recombinant Human EPHA2 extracellular domain
Lane 2 : Recombinant Human EPHA2 cytoplasmic domain
Loading : 20 ng
Dilution : 1:1000
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GTX57687 WB Image
WB analysis of PC-3 whole cell lysate using GTX57687 EphA2 antibody.
Loading : 40 μg
Dilution : 1:1000
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REFERENCE
There are currently no references for EphA2 antibody [AT66G9] (GTX57687). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EphA2 antibody [AT66G9] (GTX57687). Be the first to share your experience with this product.
![Anti-EphA2 antibody [AT66G9] used in Western Blot (WB). GTX57687](/upload/website/prouct_img/small/GTX57687/GTX57687_20200204_WB2_w_23061123_739.webp "Anti-EphA2 antibody [AT66G9] used in Western Blot (WB). GTX57687")
![Anti-EphA2 antibody [AT66G9] used in Western Blot (WB). GTX57687](/upload/website/prouct_img/small/GTX57687/GTX57687_20200204_WB1_w_23061123_866.webp "Anti-EphA2 antibody [AT66G9] used in Western Blot (WB). GTX57687")
![Anti-EphA2 antibody [AT66G9] used in Western Blot (WB). GTX57687](/upload/website/prouct_img/normal/GTX57687/GTX57687_20200204_WB2_w_23061123_739.webp "Anti-EphA2 antibody [AT66G9] used in Western Blot (WB). GTX57687")
![Anti-EphA2 antibody [AT66G9] used in Western Blot (WB). GTX57687](/upload/website/prouct_img/normal/GTX57687/GTX57687_20200204_WB1_w_23061123_866.webp "Anti-EphA2 antibody [AT66G9] used in Western Blot (WB). GTX57687")