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Epiregulin antibody

Anti-Epiregulin antibody used in Western Blot (WB). GTX16256
Anti-Epiregulin antibody used in Flow cytometry (FACS). GTX16256
Anti-Epiregulin antibody used in IHC (Paraffin sections) (IHC-P). GTX16256
Anti-Epiregulin antibody used in IHC (Paraffin sections) (IHC-P). GTX16256
Anti-Epiregulin antibody used in Western Blot (WB). GTX16256
Anti-Epiregulin antibody used in Western Blot (WB). GTX16256
Anti-Epiregulin antibody used in Western Blot (WB). GTX16256

Cat. No. GTX16256

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all Epiregulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:10-1:50
FACS 1:25
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 137-165 amino acids from the C-terminal region of human EREG.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epiregulin , EPR , ER , Ep

Background

This gene encodes a secreted peptide hormone and member of the epidermal growth factor (EGF) family of proteins. The encoded protein is a ligand of the epidermal growth factor receptor (EGFR) and the structurally related erb-b2 receptor tyrosine kinase 4 (ERBB4). The encoded protein may be involved in a wide range of biological processes including inflammation, wound healing, oocyte maturation, and cell proliferation. Additionally, the encoded protein may promote the progression of cancers of various human tissues. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES

Anti-Epiregulin antibody used in Western Blot (WB). GTX16256

GTX16256 WB Image

WB analysis of U-87 MG whole cell lysate using GTX16256 Epiregulin antibody.
Loading : 20 μg per lane
Dilution : 1:2000

Anti-Epiregulin antibody used in Flow cytometry (FACS). GTX16256

GTX16256 FACS Image

FACS analysis of HepG2 cells using GTX16256 Epiregulin antibody.
Red : Epiregulin
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

Anti-Epiregulin antibody used in IHC (Paraffin sections) (IHC-P). GTX16256

GTX16256 IHC-P Image

IHC-P analysis of human brain tissue using GTX16256 Epiregulin antibody.
Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6)
Dilution : 1:25

Anti-Epiregulin antibody used in IHC (Paraffin sections) (IHC-P). GTX16256

GTX16256 IHC-P Image

IHC-P analysis of human brain tissue using GTX16256 Epiregulin antibody.
Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6)
Dilution : 1:25

Anti-Epiregulin antibody used in Western Blot (WB). GTX16256

GTX16256 WB Image

WB analysis of various samples using GTX16256 Epiregulin antibody.
Lane 1: A549 whole cell lysates
Lane 2: human placenta lysates
Loading : 20 μg per lane
Dilution : 1:1000

Anti-Epiregulin antibody used in Western Blot (WB). GTX16256

GTX16256 WB Image

WB analysis of A549 whole cell lysate using GTX16256 Epiregulin antibody.
Loading : 20 μg per lane
Dilution : 1:2000

Anti-Epiregulin antibody used in Western Blot (WB). GTX16256

GTX16256 WB Image

WB analysis of various samples using GTX16256 Epiregulin antibody.
Lane 1: HepG2 whole cell lysates
Lane 2: A549 whole cell lysates
Lane 3: NCI-H1299 whole cell lysates
Lane 4: U-87 MG whole cell lysates
Loading : 20 μg per lane
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399