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Esrp-1/2 antibody [23A7.C9]

Anti-Esrp-1/2 antibody [23A7.C9] used in Western Blot (WB). GTX48760
Anti-Esrp-1/2 antibody [23A7.C9] used in Western Blot (WB). GTX48760

Cat. No. GTX48760

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 23A7.C9

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Mouse
Package
50 μg ($399)
See all ESRP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ELISA 1:750000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Full length recombinant mouse Esrp-1 fusion protein.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Epithelial splicing regulatory protein-1 (Esrp-1) and Esrp-2 are mRNA splicing factors that regulate the formation of epithelial cell-specific isoforms. They specifically regulate the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 and -2 act by directly binding specific sequences in mRNAs. They bind the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

DATA IMAGES

Anti-Esrp-1/2 antibody [23A7.C9] used in Western Blot (WB). GTX48760

GTX48760 WB Image

Anti-Esrp-1/2 antibody by western blot shows detection in 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 μg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.

Anti-Esrp-1/2 antibody [23A7.C9] used in Western Blot (WB). GTX48760

GTX48760 WB Image

WB analysis of transfected 293T whole cell lysate using GTX48760 Esrp-1/2 antibody [23A7.C9].
Lane 1 : GFP
Lane 2 : Espr-1
Lane 3 : Esrp-2
Loading : 5 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399