Estrogen Receptor alpha antibody [ER506]

Cat. No. GTX34710

Host	Mouse
Clonality	Monoclonal
Clone Name	ER506
Isotype	IgG1
Application	WB, IHC-P, FACS, Protein Array
Reactivity	Human
Package	100 μg ($529)

See all Estrogen Receptor alpha products

Datasheet
Document

View product citations for antibody GTX34710 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
IHC-P	Assay dependent
FACS	1-2μg/10⁶ cells
Protein Array	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Product Note

This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 2-185) of human Estrogen Receptor alpha protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ER , ESR , ESR1 , ESRA , ESTRR , Era , NR3A1 , estrogen receptor 1 , ER alpha , Estrogen Receptor alpha

Cellular Localization

Nucleus,Cytoplasm,Cell membrane,Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Gene ID: 2099 ESR1
UniProt: P03372 ESR1

Research Area

DATA IMAGES

GTX34710 WB Image

WB analysis of MCF-7 cell lysate using GTX34710 Estrogen Receptor alpha antibody [ER506].

GTX34710 FACS Image

FACS analysis of MCF-7 cells using GTX34710 Estrogen Receptor alpha antibody [ER506].
Black : Blank
Green : Isotype Control
Red : Primary antibody

GTX34710 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor, alpha Mouse Monoclonal Antibody (ER506). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

GTX34710 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX34710 Estrogen Receptor alpha antibody [ER506].

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty