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FADD antibody [J1D2]

Anti-FADD antibody [J1D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57546
Anti-FADD antibody [J1D2] used in Western Blot (WB). GTX57546
Anti-FADD antibody [J1D2] used in Western Blot (WB). GTX57546
Anti-FADD antibody [J1D2] used in IHC (Paraffin sections) (IHC-P). GTX57546

Cat. No. GTX57546

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 J1D2

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all FADD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone J1D2 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FADD protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fas associated via death domain , FADD , GIG3 , MORT1

Background

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FADD antibody [J1D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57546

GTX57546 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57546 FADD antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-FADD antibody [J1D2] used in Western Blot (WB). GTX57546

GTX57546 WB Image

WB analysis of various samples using GTX57546 FADD antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : Raw264.7 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : Ramos whole cell lysate
Lane 6 : Raji whole cell lysate
Lane 7 : Balb/3T3 whole cell lysate
Loading : 40 μg
Dilution : 1:500

Anti-FADD antibody [J1D2] used in Western Blot (WB). GTX57546

GTX57546 WB Image

WB analysis of Jurkat whole cell lysate using GTX57546 FADD antibody.
Loading : 30 μg
Dilution : 1:500

Anti-FADD antibody [J1D2] used in IHC (Paraffin sections) (IHC-P). GTX57546

GTX57546 IHC-P Image

IHC-P analysis of human breast cancer using GTX57546 FADD antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399