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FANCA antibody

Anti-FANCA antibody used in Western Blot (WB). GTX25063
Anti-FANCA antibody used in Western Blot (WB). GTX25063

Cat. No. GTX25063

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
100 μg ($589)
See all FANCA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ELISA 1:15000-1:30000
Not tested in other applications.

Calculated MW

163 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acids 995-1009 of human FANCA protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FA complementation group A , FA , FA-H , FA1 , FAA , FACA , FAH , FANCH

Cellular Localization

Nucleus,Cytoplasm

Background

FANCA is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It may be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

Database

Research Area

DATA IMAGES

Anti-FANCA antibody used in Western Blot (WB). GTX25063

GTX25063 WB Image

Western blot using GeneTex's affinity purified anti-FANCA antibody shows detection of a band at ~133 kDa (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates. The identity of the lower molecular weight bands is unknown but may represent breakdown products. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye™800 fluorescence images were captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-FANCA antibody used in Western Blot (WB). GTX25063

GTX25063 WB Image

WB analysis of HeLa whole cell lysate using GTX25063 FANCA antibody.
Loading : 35 μg
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589