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FAP antibody

Anti-FAP antibody used in Western Blot (WB). GTX48711
Anti-FAP antibody used in ELISA (ELISA). GTX48711

Cat. No. GTX48711

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Mouse
Package
50 μg ($399)
See all FAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:10000-1:20000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to an internal region of mouse FAP protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibroblast activation protein , SIMP

Cellular Localization

Cell membrane,Membrane

Background

Fibroblast Activation Protein (FAP) is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules. FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.

Database

Research Area

DATA IMAGES

Anti-FAP antibody used in Western Blot (WB). GTX48711

GTX48711 WB Image

Western blot using GeneTex's affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000.

Anti-FAP antibody used in ELISA (ELISA). GTX48711

GTX48711 ELISA Image

ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX48711 FAP antibody.
Coating : 0.1 μg

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399