GeneTex
United States (US)

FBXL10 antibody [C3], C-term

GTX104868_39708_WB_M_Brain_19011506_409.jpg
GTX104868_39708_20160518_IHC-P_R_19011506_613.jpg
GTX104868_WB_19011506_447.jpg

Cat No. GTX104868

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

153 kDa. ( Note )

Positive Control

A549 , HeLa , mouse brain

Predict Reactivity

(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FBXL10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CXXC2 antibody, FBXL10 antibody, Fbl10 antibody, JHDM1B antibody, PCCX2 antibody, KDM2B antibody, F-box and leucine-rich repeat protein 10 antibody, CXXC-type zinc finger protein 2 antibody, jmjC domain-containing histone demethylation protein 1B antibody, F-box/LRR-repeat protein 10 antibody, protein-containing CXXC domain 2 antibody, F-box protein FBL10 antibody, jumonji C domain-containing histone demethylase 1B antibody, lysine-specific demethylase 2B antibody, protein containing CXXC domain 2 antibody, jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody, [Histone-H3]-lysine-36 demethylase 1B antibody, "JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein antibody", lysine (K)-specific demethylase 2B antibody

Cellular Localization

Nucleus , nucleolus

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX104868_39708_WB_M_Brain_19011506_409.jpg

GTX104868 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX104868 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104868_39708_20160518_IHC-P_R_19011506_613.jpg

GTX104868 IHC-P Image

FBXL10 antibody [C3], C-term detects FBXL10 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
FBXL10 antibody [C3], C-term (GTX104868) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104868_WB_19011506_447.jpg

GTX104868 WB Image

Sample (30 µg of whole cell lysate)
A: A549
B: HeLa
5% SDS PAGE
GTX104868 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf