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FBXL16 antibody [N2C3]

Anti-FBXL16 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX120503
Anti-FBXL16 antibody [N2C3] used in Western Blot (WB). GTX120503
Anti-FBXL16 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120503

Cat. No. GTX120503

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all FBXL16 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Mouse, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FBXL16. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732124

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F-box and leucine rich repeat protein 16 , C16orf22 , Fbl16 , c380A1.1

Background

Members of the F-box protein family, such as FBXL16, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FBXL16 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX120503

GTX120503 IHC-P Image

FBXL16 antibody [N2C3] detects FBXL16 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
FBXL16 antibody [N2C3] (GTX120503) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FBXL16 antibody [N2C3] used in Western Blot (WB). GTX120503

GTX120503 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FBXL16 antibody [N2C3] (GTX120503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FBXL16 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120503

GTX120503 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using FBXL16(GTX120503) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169