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FBXO32 antibody

Orthogonal
Anti-FBXO32 antibody used in Western Blot (WB). GTX47819
Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819
Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819
Anti-FBXO32 antibody used in Western Blot (WB). GTX47819
Anti-FBXO32 antibody used in Immunohistochemistry (IHC). GTX47819
Anti-FBXO32 antibody used in Western Blot (WB). GTX47819

Cat. No. GTX47819

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA, IHC

Reactivity

 Mouse, Rat
Package
100 μg ($449)
See all FBXO32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
IHC-P Assay dependent
IP 1:200
ELISA 1:10000
IHC 1:50-1:150
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine, 0.5% BSA, 30% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64-0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide taken within amino acid region 240-280 on Atr1 protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_11173218

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F-box protein 32 , Fbx32 , MAFbx

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-FBXO32 antibody used in Western Blot (WB). GTX47819

GTX47819 WB Image

WB analysis of Fbx32 recombinant protein using GTX47819 FBXO32 antibody.
Dilution : 1:500

Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819

GTX47819 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX47819 FBXO32 antibody.
Dilution : 1:50

Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819

GTX47819 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX47819 FBXO32 antibody.
Dilution : 1:50

Anti-FBXO32 antibody used in Western Blot (WB). GTX47819

GTX47819 WB Image

WB analysis of mouse heart tissue lysate using GTX47819 FBXO32 antibody.
Dilution : 1:500

Anti-FBXO32 antibody used in Immunohistochemistry (IHC). GTX47819

GTX47819 IHC Image

Orthogonal

The data was published in the journal Biomed Res Int in 2018. PMID: 29992158

Anti-FBXO32 antibody used in Western Blot (WB). GTX47819

GTX47819 WB Image

Orthogonal

The data was published in the journal Biomed Res Int in 2018. PMID: 29992158

REFERENCE

Application Reference

Shi Z et al. Biomed Res Int 2018; 2018 : 6947612 Condylar Degradation from Decreased Occlusal Loading following Masticatory Muscle Atrophy.
Zhan Shi et al. BioMed Research International 2018; 2018 : Article ID 6947612 Condylar Degradation from Decreased Occlusal Loading following Masticatory Muscle Atrophy.
Application : IHC-P
Reactivity : Rat
Submit a Reference

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Package List Price ($)
$ 449