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FCP1 antibody [N1N2], N-term

Anti-FCP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115194
Anti-FCP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115194

Cat. No. GTX115194

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FCP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Zebrafish, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human FCP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CTD phosphatase subunit 1 , CCFDN , FCP1

Cellular Localization

Nucleus

Background

This gene encodes a protein which interacts with the carboxy-terminus of transcription initiation factor TFIIF, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. The protein may also represent a component of an RNA polymerase II holoenzyme complex. Alternative splicing of this gene results in two transcript variants encoding 2 different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FCP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115194

GTX115194 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX115194 diluted at 1:1000

Anti-FCP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115194

GTX115194 ICC/IF Image

FCP1 antibody [N1N2], N-term detects FCP1 protein at nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FCP1 protein stained by FCP1 antibody [N1N2], N-term (GTX115194) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169