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FEM1C antibody

Anti-FEM1C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121493
Anti-FEM1C antibody used in Western Blot (WB). GTX121493

Cat. No. GTX121493

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FEM1C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human FEM1C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168151

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fem-1 homolog C , EUROIMAGE686608 , EUROIMAGE783647 , FEM1A

Cellular Localization

Cytoplasm

Background

Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.

Database

Research Area

DATA IMAGES

Anti-FEM1C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121493

GTX121493 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FEM1C(GTX121493) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-FEM1C antibody used in Western Blot (WB). GTX121493

GTX121493 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX121493 diluted at 1:5000

REFERENCE

Application Reference

Dankert JF et al. Cell Cycle 2017; : 0 FEM1 proteins are ancient regulators of SLBP degradation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169