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FEN1 antibody

Anti-FEN1 antibody used in Western Blot (WB). GTX70257

Cat. No. GTX70257

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all FEN1 products

APPLICATION

Application Note

For WB: Use at a dilution of 1:2,500. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

43 kDa. ( Note )

Positive Control

HeLa, Raji, 3T3

PROPERTIES

Form

Liquid

Buffer

Serum diluted with PBS pH7.4

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human FEN-1 protein

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_370524

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

flap structure-specific endonuclease 1 , FEN-1 , MF1 , RAD2

Cellular Localization

nucleoplasm

Background

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FEN1 antibody used in Western Blot (WB). GTX70257

GTX70257 WB Image

Western blot analysis of FEN1 expression in Raji (A) and NIH-3T3 (B) whole cell lysates.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399