APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-2μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX95551-PEP).
Product Note
This antibody is expected to recognise all three reported isoforms (NP_001036194.1; NP_001036212.1; NP_005239.1).
Predict Reactivity
Human, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-TSAEPQYQPGDQT, from the C Terminus of the protein sequence according to NP_001036194.1; NP_001036212.1; NP_005239.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
FGR proto-oncogene, Src family tyrosine kinase , SRC2 , c-fgr , c-src2 , p55-Fgr , p55c-fgr , p58-Fgr , p58c-fgr
Cellular Localization
Cell membrane , Cytoplasm, cytosol , Cytoplasm, cytoskeleton , Mitochondrion inner membrane
Background
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX95551 WB Image
WB analysis of mouse spleen extracts using GTX95551 FGR antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for FGR antibody, C-term (GTX95551). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FGR antibody, C-term (GTX95551). Be the first to share your experience with this product.
