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FHIT antibody

Anti-FHIT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31890
Anti-FHIT antibody used in Western Blot (WB). GTX31890

Cat. No. GTX31890

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all FHIT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

FHIT antibody was raised against an 18 amino acid peptide near the carboxy terminus of human FHIT.The immunogen is located within the last 50 amino acids of FHIT .

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fragile histidine triad , AP3Aase , FRA3B

Cellular Localization

Cytoplasm,Mitochondrion,Nucleus

Background

This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-FHIT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31890

GTX31890 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31890 FHIT antibody.
Working concentration : 5 μg/ml

Anti-FHIT antibody used in Western Blot (WB). GTX31890

GTX31890 WB Image

WB analysis of HeLa cell lysate using GTX31890 FHIT antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399