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FOXA3 antibody

Orthogonal
Anti-FOXA3 antibody used in Western Blot (WB). GTX79266
Anti-FOXA3 antibody used in ChIP assay (ChIP assay). GTX79266

Cat. No. GTX79266

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all FOXA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:1000
ChIP assay 1-3 μl
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues S(4) V K M E A H D L A E W S Y Y P E A G E(23) of mouse FOXA3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11170543

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box A3 , Hnf-3g , Hnf3g , Tcf-3g , Tcf3g

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-FOXA3 antibody used in Western Blot (WB). GTX79266

GTX79266 WB Image

WB analysis of 25 ug of HepG2 (lane 1) mouse liver (lane 2) and rat liver (lane 3) lysates using GTX79266 FOXA3 antibody.
Dilution : 1:500

Anti-FOXA3 antibody used in ChIP assay (ChIP assay). GTX79266

GTX79266 ChIP assay Image

Orthogonal

ChIP analysis of 1x10⁶ HCT116 colon carcinoma cells treated with serum for 0, 15, and 30 minutes using GTX79266 FOXA3 antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1.
IP reaction : 1.0ul antibody / 100ul sample

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399