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FOXE1 antibody

Anti-FOXE1 antibody used in Immunoprecipitation (IP). GTX100275
Anti-FOXE1 antibody used in Western Blot (WB). GTX100275

Cat. No. GTX100275

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FOXE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240827

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box E1 , FKHL15 , FOXE2 , HFKH4 , HFKL5 , NMTC4 , TITF2 , TTF-2 , TTF2

Cellular Localization

Nucleus

Background

This intronless gene belongs to the forkhead family of transcription factors, which is characterized by a distinct forkhead domain. This gene functions as a thyroid transcription factor which likely plays a crucial role in thyroid morphogenesis. Mutations in this gene are associated with congenital hypothyroidism and cleft palate with thyroid dysgenesis. The map localization of this gene suggests it may also be a candidate gene for squamous cell epithelioma and hereditary sensory neuropathy type I. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FOXE1 antibody used in Immunoprecipitation (IP). GTX100275

GTX100275 IP Image

FOXE1 antibody immunoprecipitates FOXE1 protein in IP experiments.
IP Sample: HeLa whole cell lysate/extract
A. 40 μg HeLa whole cell lysate/extract
B. Control with 2 μg of preimmune rabbit IgG
C. Immunoprecipitation of FOXE1 protein by 2 μg of FOXE1 antibody (GTX100275)
15% SDS-PAGE
The immunoprecipitated FOXE1 protein was detected by FOXE1 antibody (GTX100275) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Anti-FOXE1 antibody used in Western Blot (WB). GTX100275

GTX100275 WB Image

Sample (30 ug of whole cell lysate)
A: HeLaS3
10% SDS PAGE
GTX100275 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169