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FRA1 antibody

Comparison Orthogonal
Anti-FRA1 antibody used in Western Blot (WB). GTX134242
Anti-FRA1 antibody used in Western Blot (WB). GTX134242
Anti-FRA1 antibody used in Western Blot (WB). GTX134242

Cat. No. GTX134242

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FRA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

NIH-3T3 nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of rat FRA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887245

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FOS like 1, AP-1 transcription factor subunit , Fra-1 , fra-1

Cellular Localization

Nucleus

Background

protein, antigenically related to c-fos; involved in serum inducible cellular immediate-early transcriptional response [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-FRA1 antibody used in Western Blot (WB). GTX134242

GTX134242 WB Image

Comparison Orthogonal

NIH-3T3 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with FRA1 antibody (GTX134242) diluted at 1:2000 and competitor's antibody (sc-605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-FRA1 antibody used in Western Blot (WB). GTX134242

GTX134242 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FRA1 antibody (GTX134242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FRA1 antibody used in Western Blot (WB). GTX134242

GTX134242 WB Image

Orthogonal

Untreated (–) and treated (+) Rat-2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FRA1 antibody (GTX134242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Application : WB
Liu Y et al. Pharmacol Res 2021; 165 : 105405 FOSL1 promotes tumorigenesis in colorectal carcinoma by mediating the FBXL2/Wnt/β-catenin axis via Smurf1.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169