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FRK antibody

Anti-FRK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107456
Anti-FRK antibody used in IHC (Paraffin sections) (IHC-P). GTX107456
Anti-FRK antibody used in Western Blot (WB). GTX107456

Cat. No. GTX107456

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FRK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FRK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036965

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fyn related Src family tyrosine kinase , GTK , PTK5 , RAK

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FRK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107456

GTX107456 ICC/IF Image

FRK antibody detects FRK protein at Cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min.
Green: FRK protein stained by FRK antibody (GTX107456) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-FRK antibody used in IHC (Paraffin sections) (IHC-P). GTX107456

GTX107456 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using FRK(GTX107456) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FRK antibody used in Western Blot (WB). GTX107456

GTX107456 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
FRK antibody
GTX107456 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169