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FUBP1 antibody

Orthogonal
Anti-FUBP1 antibody used in Western Blot (WB). GTX104579
Anti-FUBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104579
Anti-FUBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104579
Anti-FUBP1 antibody used in Western Blot (WB). GTX104579
Anti-FUBP1 antibody used in Immunoprecipitation (IP). GTX104579
Anti-FUBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104579
Anti-FUBP1 antibody used in Western Blot (WB). GTX104579

Cat. No. GTX104579

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FUBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FUBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165485

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

far upstream element binding protein 1 , FBP , FUBP , hDH V

Cellular Localization

Nucleus

Background

This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FUBP1 antibody used in Western Blot (WB). GTX104579

GTX104579 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX104579) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104579

GTX104579 IHC-P Image

FUBP1 antibody detects FUBP1 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FUBP1 antibody (GTX104579) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FUBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104579

GTX104579 IHC-P Image

FUBP1 antibody detects FUBP1 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FUBP1 antibody (GTX104579) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FUBP1 antibody used in Western Blot (WB). GTX104579

GTX104579 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX104579) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUBP1 antibody used in Immunoprecipitation (IP). GTX104579

GTX104579 IP Image

FUBP-1 antibody immunoprecipitates FUBP-1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 μg of FUBP-1 antibody (GTX104579) 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody (GTX1104579) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-FUBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104579

GTX104579 ICC/IF Image

FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FUBP1 protein stained by FUBP1 antibody (GTX104579) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-FUBP1 antibody used in Western Blot (WB). GTX104579

GTX104579 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX104579) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang J et al. mBio 2020; 11 (2) RNA Binding Motif Protein RBM45 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19 through Binding to Novel Intron Splicing Enhancers.
Reactivity : Human
Kralovicova J et al. Nucleic Acids Res 2016; Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons.
Application : WB
Reactivity : Human
Kralovicova J et al. Sci Rep 2016; 6 : 18741 Exon-centric regulation of ATM expression is population-dependent and amenable to antisense modification by pseudoexon targeting.
Miro J et al. Nucleic Acids Res 2015; 43 (4) : 2378-89 FUBP1: a new protagonist in splicing regulation of the DMD gene.
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169