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FUS2 antibody [AT2F4]

Anti-FUS2 antibody [AT2F4] used in Western Blot (WB). GTX50012
Anti-FUS2 antibody [AT2F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50012

Cat. No. GTX50012

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT2F4

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all FUS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant FUS2 (1-308aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Vertebrate FUS2 genes, which are known to be putative tumor suppressor gene, contain several important domains such as the catalytic N-acetyltransferase (NAT) domain. NAT domain is essential enzymes involved in several sophisticated cellular processes such as N-acetylation, O-acetylatin. NAT enzymes may be involved in susceptibility to cancer including colorectal cancer because of the presence of carcinogenic heterocyclic amines in some cooked foods. FUS2 was physically localized to the cytoplasm. Also, FUS2 showed the actin dependent movement, closely related to the polarization in the budding yeast, Saccharomyces cerevisiae.

Research Area

DATA IMAGES

Anti-FUS2 antibody [AT2F4] used in Western Blot (WB). GTX50012

GTX50012 WB Image

Cell lysates of NIH3T3(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FUS2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-FUS2 antibody [AT2F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50012

GTX50012 ICC/IF Image

Immunofluorescence of human A549 cells stained with Hoechst 33342 (Blue) and monoclonal anti-human FUS2 antibody (1:500) with Texas Red (red).

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399