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Ferrochelatase antibody [N2C3]

Anti-Ferrochelatase antibody [N2C3] used in Western Blot (WB). GTX113435

Cat. No. GTX113435

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Ferrochelatase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ferrochelatase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036914

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ferrochelatase , EPP , EPP1 , FCE

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ferrochelatase antibody [N2C3] used in Western Blot (WB). GTX113435

GTX113435 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ferrochelatase antibody [N2C3] (GTX113435) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 19965627

REFERENCE

Application Reference

Ast T et al. Cell 2019; 177 (6) : 1507-1521.e16 Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.
Yang DF et al. J Formos Med Assoc. 2014; 113 (2) : 88-93 * enhances *-mediated photodynamic therapy-induced killing of human scc4 cells by upregulation of coproporphyrinogen oxidase.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169