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Ferroportin 1 antibody

Anti-Ferroportin 1 antibody used in Western Blot (WB). GTX54821
Anti-Ferroportin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54821
Anti-Ferroportin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54821

Cat. No. GTX54821

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all Ferroportin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)EGTHLMGVKDSNIHE, corresponding to amino acid residues 261-275 (2nd  intracellular loop ; Note: the st ructure of Ferroportin is st ill controversial and there is no defined topology yet) of human Ferroportin (Accession  : Q9NP59).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 40 member 1 , FPN1 , HFE4 , IREG1 , MST079 , MSTP079 , MTP1 , SLC11A3

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a cell membrane protein that may be involved in iron export from duodenal epithelial cells. Defects in this gene are a cause of hemochromatosis type 4 (HFE4). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ferroportin 1 antibody used in Western Blot (WB). GTX54821

GTX54821 WB Image

WB analysis of rat spleen (lanes 1 and 5), rat small intestine (lanes 2 and 6), rat liver (lanes 3 and 7), and mouse liver (lanes 4 and 8) lysates using GTX54821 Ferroportin 1 antibody preincubated with or without immunogen peptide.
Dilution : 1:500

Anti-Ferroportin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54821

GTX54821 IHC-Fr Image

IHC-Fr analysis of mouse brain tissue using GTX54821 Ferroportin 1 antibody. Cell nuclei are stained using DAPI (blue).
Panel A : In the cerebellum, Ferroportin (green) appears in cerebellar purkinje cells (horizontal arrows) and their dendrites (vertical arrows).
Panel B : In the forebrain, intense Ferroportin appears (green) in oligodendrocytes (horizontal arrows) and moderate staining is detected in neurons (vertical arrow).

Anti-Ferroportin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54821

GTX54821 IHC-Fr Image

IHC-Fr analysis of mouse brain tissue using GTX54821 Ferroportin 1 antibody. Cell nuclei are stained using DAPI (blue).
Panel A : Ferroportin staining (green) appears in several cells in the SNC.
Panel B : Tyrosine hydroxylase (red) stains dopaminergic neurons.
Panel C : Merge of A and B demonstrates Ferroportin in dopaminergic and non-dopaminergic neurons.

REFERENCE

Application Reference

LT Tsai et al. Antioxidants (Basel) 2023; 12 (11) : 1931 Inhibition of Indoxyl Sulfate-Induced Reactive Oxygen Species-Related Ferroptosis Alleviates Renal Cell Injury In Vitro and Chronic Kidney Disease Progression In Vivo
Application : WB
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639