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Firefly Luciferase antibody

Anti-Firefly Luciferase antibody used in Western Blot (WB). GTX125850

Cat. No. GTX125850

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB
Package
100 μl ($399),
25 μl ($169)
See all Firefly Luciferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T transfected Renilla Luciferase

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of Firefly Luciferase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173191

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fire fly Luciferase , Luciferase , Photinus pyralis luciferase , Firefly luciferase

Research Area

DATA IMAGES

Anti-Firefly Luciferase antibody used in Western Blot (WB). GTX125850

GTX125850 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: 293T transfected firefly
C: 293T transfected Renilla Luciferase
10% SDS PAGE
GTX125850 diluted at 1:3000

REFERENCE

Application Reference

CC Liao et al. Research Square 2024; G-cleave LC3B Biosensor: Monitoring Autophagy and Assessing Resveratrol's Synergistic Impact on Doxorubicin-Induced Apoptosis in Breast Cancer Cells
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169