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Flightless I antibody [N3C1], Internal

Anti-Flightless I antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106269
Anti-Flightless I antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106269
Anti-Flightless I antibody [N3C1], Internal used in Western Blot (WB). GTX106269
Anti-Flightless I antibody [N3C1], Internal used in Western Blot (WB). GTX106269

Cat. No. GTX106269

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Flightless I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

145 kDa. ( Note )

Positive Control

Mouse brain , Raji

Predict Reactivity

Rat, Zebrafish, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Flightless I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950289

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FLII actin remodeling protein , FLI , FLIL , Fli1

Cellular Localization

Nucleus , Cytoplasm , cytoskeleton , Centrosome

Background

This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Flightless I antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106269

GTX106269 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I(GTX106269) antibody at 1:200 dilution.

Anti-Flightless I antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106269

GTX106269 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless I(GTX106269) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Flightless I antibody [N3C1], Internal used in Western Blot (WB). GTX106269

GTX106269 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX106269 diluted at 1:1000

Anti-Flightless I antibody [N3C1], Internal used in Western Blot (WB). GTX106269

GTX106269 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
5% SDS PAGE
GTX106269 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169