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Flotillin 1 antibody

Anti-Flotillin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54027
Anti-Flotillin 1 antibody used in Western Blot (WB). GTX54027
Anti-Flotillin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54027
Anti-Flotillin 1 antibody used in Immunoprecipitation (IP). GTX54027
Anti-Flotillin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54027

Cat. No. GTX54027

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Flotillin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 128-427 of human FLOT1 (NP_005794.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

flotillin 1

Cellular Localization

Endosome

Background

This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Flotillin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54027

GTX54027 IHC-P Image

IHC-P analysis of rat heart tissue using GTX54027 Flotillin 1 antibody.
Dilution : 1:100

Anti-Flotillin 1 antibody used in Western Blot (WB). GTX54027

GTX54027 WB Image

WB analysis of various sample lysates using GTX54027 Flotillin 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Flotillin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54027

GTX54027 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54027 Flotillin 1 antibody.
Dilution : 1:100

Anti-Flotillin 1 antibody used in Immunoprecipitation (IP). GTX54027

GTX54027 IP Image

IP analysis of HeLa cell lysate using GTX54027 Flotillin 1 antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

Anti-Flotillin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54027

GTX54027 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54027 Flotillin 1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399