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Frataxin antibody

Anti-Frataxin antibody used in Western Blot (WB). GTX54036
Anti-Frataxin antibody used in IHC (Paraffin sections) (IHC-P). GTX54036
Anti-Frataxin antibody used in IHC (Paraffin sections) (IHC-P). GTX54036
Anti-Frataxin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54036
Anti-Frataxin antibody used in IHC (Paraffin sections) (IHC-P). GTX54036

Cat. No. GTX54036

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Frataxin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN (NP_000135.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887836

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

frataxin , CyaY , FA , FARR , FRDA , X25

Cellular Localization

Mitochondrion,Cytoplasm, cytosol

Background

This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-Frataxin antibody used in Western Blot (WB). GTX54036

GTX54036 WB Image

WB analysis of various sample lysates using GTX54036 Frataxin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Frataxin antibody used in IHC (Paraffin sections) (IHC-P). GTX54036

GTX54036 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX54036 Frataxin antibody.
Dilution : 1:100

Anti-Frataxin antibody used in IHC (Paraffin sections) (IHC-P). GTX54036

GTX54036 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX54036 Frataxin antibody.
Dilution : 1:100

Anti-Frataxin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54036

GTX54036 ICC/IF Image

ICC/IF analysis of L929 cells using GTX54036 Frataxin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Frataxin antibody used in IHC (Paraffin sections) (IHC-P). GTX54036

GTX54036 IHC-P Image

IHC-P analysis of human liver tissue using GTX54036 Frataxin antibody.
Dilution : 1:100

REFERENCE

Application Reference

Jill S Napierala et al. Mol Cell Proteomics 2021; 20 : 100094 Reverse Phase Protein Array Reveals Correlation of Retinoic Acid Metabolism With Cardiomyopathy in Friedreich's Ataxia.
Application : WB
Reactivity : Human
Fil D et al. Dis Model Mech 2020; 13 (7) Mitochondrial damage and senescence phenotype of cells derived from a novel frataxin G127V point mutation mouse model of Friedreich's ataxia.
Li J et al. Hum Gene Ther 2020; 31 (15-16) : 839-851 Defining Transcription Regulatory Elements in the Human Frataxin Gene: Implications for Gene Therapy.
Application : WB
Reactivity : Human
Zhao H et al. Sci Rep 2020; 10 (1) : 6095 Long-term voluntary running prevents the onset of symptomatic Friedreich's ataxia in mice.
Application : WB
Reactivity : Mouse
Ast T et al. Cell 2019; 177 (6) : 1507-1521.e16 Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399