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Frizzled 3 antibody

Orthogonal
Anti-Frizzled 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100182
Anti-Frizzled 3 antibody used in Western Blot (WB). GTX100182
Anti-Frizzled 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100182

Cat. No. GTX100182

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Frizzled 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human Frizzled 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240839

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

frizzled class receptor 3 , Fz-3

Cellular Localization

Membrane; Multi-pass membrane protein

Background

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-Frizzled 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100182

GTX100182 IHC-P Image

Frizzled 3 antibody detects Frizzled 3 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Frizzled 3 stained by Frizzled 3 antibody (GTX100182) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Frizzled 3 antibody used in Western Blot (WB). GTX100182

GTX100182 WB Image

Orthogonal

The data was published in the journal PLoS One in 2013.PMID: 23472152

Anti-Frizzled 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100182

GTX100182 IHC-P Image

The data was published in the journal PLoS One in 2015. PMID: 25860837

REFERENCE

Application Reference

Liu W et al. Theranostics 2018; 8 (20) : 5575-5592 Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3帣 Complex in Hypophosphatasia.
Mine M et al. PLoS One 2015; 10 (7) : e0131350 miR-203 Inhibits Frizzled-2 Expression via CD82/KAI1 Expression in Human Lung Carcinoma Cells.
Application : WB
Reactivity : Human
Iizuka-Kogo A et al. PLoS One 2015; 10 (4) : e0123965 Requirement of DLG1 for cardiovascular development and tissue elongation during cochlear, enteric, and skeletal development: possible role in convergent extension.
Application : IHC
Reactivity : Mouse
Chen CC et al. Am J Respir Crit Care Med 2014; 190 (4) : 433-44 Shisa3 Is Associated with Prolonged Survival through Promoting β-Catenin Degradation in Lung Cancer.
Application : WB
Reactivity : Human
Chan YT et al. PLoS One 2013; 8 (3) : e58169 Concordant and discordant regulation of target genes by miR-31 and its isoforms.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169