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Frizzled 8 antibody

Anti-Frizzled 8 antibody used in Western Blot (WB). GTX104679
Anti-Frizzled 8 antibody used in Western Blot (WB). GTX104679
Anti-Frizzled 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX104679

Cat. No. GTX104679

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Frizzled 8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rat, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Frizzled 8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240840

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

frizzled class receptor 8 , FZ-8 , hFZ8

Background

This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Frizzled 8 antibody used in Western Blot (WB). GTX104679

GTX104679 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX104679 diluted at 1:10000
Anti-Frizzled 8 antibody used in Western Blot (WB). GTX104679

GTX104679 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 8 antibody (GTX104679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Frizzled 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX104679

GTX104679 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Frizzled 8(GTX104679) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Mine M et al. PLoS One 2015; 10 (7) : e0131350 miR-203 Inhibits Frizzled-2 Expression via CD82/KAI1 Expression in Human Lung Carcinoma Cells.
Application : WB
Reactivity : Human
Chen CC et al. Am J Respir Crit Care Med 2014; 190 (4) : 433-44 Shisa3 Is Associated with Prolonged Survival through Promoting β-Catenin Degradation in Lung Cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169