APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT3F6 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Fumarase protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fumarate hydratase , FH , FMRD , HLRCC , LRCC , MCL , MCUL1 , Fumarate hydratase
Cellular Localization
Mitochondrion,Cytoplasm
Background
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Fumarate hydratase antibody [AT3F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57625](/templates/default/js/lightbox/images/loading.gif "Anti-Fumarate hydratase antibody [AT3F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57625")
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GTX57625 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57625 Fumarate hydratase antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57625 FACS Image
FACS analysis of HeLa cells using GTX57625 Fumarate hydratase antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg
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GTX57625 WB Image
WB analysis of various samples using GTX57625 Fumarate hydratase antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : LNCap whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : 293T whole cell lysate
Lane 6 : K562 whole cell lysate
Lane 7 : Jurkat whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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REFERENCE
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REVIEW
There are currently no reviews for Fumarate hydratase antibody [AT3F6] (GTX57625). Be the first to share your experience with this product.
![Anti-Fumarate hydratase antibody [AT3F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57625](/upload/website/prouct_img/small/GTX57625/GTX57625_20200204_IF_w_23061123_149.webp "Anti-Fumarate hydratase antibody [AT3F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57625")
![Anti-Fumarate hydratase antibody [AT3F6] used in Flow cytometry (FACS). GTX57625](/upload/website/prouct_img/small/GTX57625/GTX57625_20200204_FC_w_23061123_859.webp "Anti-Fumarate hydratase antibody [AT3F6] used in Flow cytometry (FACS). GTX57625")
![Anti-Fumarate hydratase antibody [AT3F6] used in Western Blot (WB). GTX57625](/upload/website/prouct_img/small/GTX57625/GTX57625_20200204_WB_w_23061123_289.webp "Anti-Fumarate hydratase antibody [AT3F6] used in Western Blot (WB). GTX57625")
![Anti-Fumarate hydratase antibody [AT3F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57625](/upload/website/prouct_img/normal/GTX57625/GTX57625_20200204_IF_w_23061123_149.webp "Anti-Fumarate hydratase antibody [AT3F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57625")
![Anti-Fumarate hydratase antibody [AT3F6] used in Flow cytometry (FACS). GTX57625](/upload/website/prouct_img/normal/GTX57625/GTX57625_20200204_FC_w_23061123_859.webp "Anti-Fumarate hydratase antibody [AT3F6] used in Flow cytometry (FACS). GTX57625")
![Anti-Fumarate hydratase antibody [AT3F6] used in Western Blot (WB). GTX57625](/upload/website/prouct_img/normal/GTX57625/GTX57625_20200204_WB_w_23061123_289.webp "Anti-Fumarate hydratase antibody [AT3F6] used in Western Blot (WB). GTX57625")