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G protein alpha 13 antibody

KO/KD Comparison
Anti-G protein alpha 13 antibody used in Western Blot (WB). GTX131045
Anti-G protein alpha 13 antibody used in Western Blot (WB). GTX131045
Anti-G protein alpha 13 antibody used in IHC (Paraffin sections) (IHC-P). GTX131045
Anti-G protein alpha 13 antibody used in IHC (Paraffin sections) (IHC-P). GTX131045

Cat. No. GTX131045

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all G protein alpha 13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G protein alpha 13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886409

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit alpha 13 , G13

Cellular Localization

Membrane; Lipid-anchor , Melanosome

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Database

Research Area

DATA IMAGES

Anti-G protein alpha 13 antibody used in Western Blot (WB). GTX131045

GTX131045 WB Image

KO/KD

Wild-type (WT) and G protein alpha 13 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with G protein alpha 13 antibody (GTX131045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G protein alpha 13 antibody used in Western Blot (WB). GTX131045

GTX131045 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with G protein alpha 13 antibody (GTX131045) diluted at 1:1000 and competitor's antibody (SC-410) diluted by 1:50.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-G protein alpha 13 antibody used in IHC (Paraffin sections) (IHC-P). GTX131045

GTX131045 IHC-P Image

G protein alpha 13 antibody detects G protein alpha 13 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
G protein alpha 13 stained by G protein alpha 13 antibody (GTX131045) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-G protein alpha 13 antibody used in IHC (Paraffin sections) (IHC-P). GTX131045

GTX131045 IHC-P Image

G protein alpha 13 antibody detects G protein alpha 13 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
G protein alpha 13 stained by G protein alpha 13 antibody (GTX131045) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169