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G protein alpha 16 antibody

Overexpression
Anti-G protein alpha 16 antibody used in Western Blot (WB). GTX118385
Anti-G protein alpha 16 antibody used in IHC (Paraffin sections) (IHC-P). GTX118385
Anti-G protein alpha 16 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118385
Anti-G protein alpha 16 antibody used in Western Blot (WB). GTX118385

Cat. No. GTX118385

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all G protein alpha 16 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

THP-1 , GNA16-transfected 293T

Predict Reactivity

Mouse, Rat, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G protein alpha 16. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618060

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit alpha 15 , GNA16

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Database

Research Area

DATA IMAGES

Anti-G protein alpha 16 antibody used in Western Blot (WB). GTX118385

GTX118385 WB Image

G protein alpha 16 antibody detects GNA15 protein by Western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
10 % SDS-PAGE
G protein alpha 16 antibody (GTX118385) dilution: 1:1000

Anti-G protein alpha 16 antibody used in IHC (Paraffin sections) (IHC-P). GTX118385

GTX118385 IHC-P Image

G protein alpha 16 antibody detects G protein alpha 16 protein at cytoplasm on CX-1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CX-1 xenograft.
G protein alpha 16 antibody (GTX118385) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-G protein alpha 16 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118385

GTX118385 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using G protein alpha 16(GTX118385) antibody at 1:500 dilution.

Anti-G protein alpha 16 antibody used in Western Blot (WB). GTX118385

GTX118385 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with G protein alpha 16 antibody (GTX118385) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169