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G3BP1 antibody

Anti-G3BP1 antibody used in Western Blot (WB). GTX112191
Anti-G3BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112191
Anti-G3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112191
Anti-G3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112191

Cat. No. GTX112191

Reference 5
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all G3BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G3BP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164802

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G3BP stress granule assembly factor 1 , G3BP , HDH-VIII

Cellular Localization

Cytoplasm , cytosol , Cell membrane , Nucleus

Background

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-G3BP1 antibody used in Western Blot (WB). GTX112191

GTX112191 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody (GTX112191) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G3BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112191

GTX112191 ICC/IF Image

G3BP1 antibody detects G3BP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: G3BP1 stained by G3BP1 antibody (GTX112191) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-G3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112191

GTX112191 IHC-P Image

G3BP1 antibody detects G3BP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
G3BP1 stained by G3BP1 antibody (GTX112191) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-G3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112191

GTX112191 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using G3BP1(GTX112191) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Y Liao et al. J Cell Biol 2024; 223 (7) : e202310006 UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope
Agote-Aran A et al. EMBO J 2020; 39 (20) : e104467 Spatial control of nucleoporin condensation by fragile X-related proteins.
Wen W et al. Front Immunol 2020; 11 : 577838 Seneca Valley Virus 3C Protease Inhibits Stress Granule Formation by Disrupting eIF4GI-G3BP1 Interaction.
Application : WB
Reactivity : Human
Zhang LN et al. J Cell Physiol 2019; (Epub) Loss of G3BP1 suppresses proliferation, migration, and invasion of esophageal cancer cells via Wnt/β-catenin and PI3K/AKT signaling pathways.
Application : WB
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

G3BP1 antibody Cat. No. GTX112191

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-G3BP1 antibody used in Western Blot (WB). GTX112191
Date : Anonymous submitted on 28-Jul-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human blood T cell
Amount used : 5 μg
Blocking : 5% milk in TBST, 4°C, 16Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169