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GABA B Receptor 1 antibody [N2C1], Internal

Anti-GABA B Receptor 1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102511
Anti-GABA B Receptor 1 antibody [N2C1], Internal used in Western Blot (WB). GTX102511
Anti-GABA B Receptor 1 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102511
Anti-GABA B Receptor 1 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102511
Anti-GABA B Receptor 1 antibody [N2C1], Internal used in Western Blot (WB). GTX102511
Anti-GABA B Receptor 1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102511
Anti-GABA B Receptor 1 antibody [N2C1], Internal used in Western Blot (WB). GTX102511

Cat. No. GTX102511

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GABA B Receptor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

PC-3 , U87-MG , SK-N-SH , C8D30

Predict Reactivity

Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human GABA B Receptor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950325

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gamma-aminobutyric acid type B receptor subunit 1 , GABABR1 , GABBR1-3 , GB1 , GPRC3A

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein , Isoform 1E: Secreted

Background

Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GABA B Receptor 1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102511

GTX102511 ICC/IF Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABA B Receptor 1 protein stained by GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:500.
Red: SOX2 protein stained by SOX2 antibody (GTX627405) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-GABA B Receptor 1 antibody [N2C1], Internal used in Western Blot (WB). GTX102511

GTX102511 WB Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABBR1 protein by Western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
7.5 % SDS-PAGE
GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) dilution: 1:500

Anti-GABA B Receptor 1 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102511

GTX102511 IHC-P Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GABA B Receptor 1 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102511

GTX102511 IHC-P Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytosol on human brain by immunohistochemical analysis.
Sample: Paraffin-embedded human brain.
GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GABA B Receptor 1 antibody [N2C1], Internal used in Western Blot (WB). GTX102511

GTX102511 WB Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABBR1 protein by Western blot analysis.
A. 30 μg PC-3 whole cell lysate/extract
B. 30 μg U87-MG whole cell lysate/extract
C. 30 μg SK-N-SH whole cell lysate/extract
7.5 % SDS-PAGE
GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) dilution: 1:500

Anti-GABA B Receptor 1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102511

GTX102511 ICC/IF Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABA B Receptor 1 protein stained by GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-GABA B Receptor 1 antibody [N2C1], Internal used in Western Blot (WB). GTX102511

GTX102511 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

L Li et al. Exp Neurol 2023; 372 : 114616 Neonatal di-(2-ethylhexyl)phthalate exposure induces permanent alterations in secretory CRH neuron characteristics in the hypothalamus paraventricular region of adult male rats
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169