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GADD153 antibody [N1C3]

KO/KD Orthogonal
Anti-GADD153 antibody [N1C3] used in Western Blot (WB). GTX112827

Cat. No. GTX112827

Reference 10
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GADD153 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human GADD153 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10726444

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA damage inducible transcript 3 , AltDDIT3 , C/EBPzeta , CEBPZ , CHOP , CHOP-10 , CHOP10 , GADD153

Cellular Localization

Nucleus

Background

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GADD153 antibody [N1C3] used in Western Blot (WB). GTX112827

GTX112827 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GADD153 antibody [N1C3] (GTX112827) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wei-Chun Chen et al. Viruses 2021; 13 (4) (E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression.
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Rauti R et al. Arch Toxicol 2020; 94 (3) : 845-855 Bilirubin disrupts calcium homeostasis in neonatal hippocampal neurons: a new pathway of neurotoxicity.
Application : WB
Reactivity : Rat
Zhu R et al. Mol Cell Endocrinol 2019; 493 : 110470 Defective endoplasmic reticulum export causes proinsulin misfolding in pancreatic β cells.
Application : WB
Reactivity : Mouse
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Submit a Reference

REVIEW

GADD153 antibody [N1C3] Cat. No. GTX112827

Rating
( Average 4 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Anti-GADD153 antibody [N1C3] used in Western Blot (WB). GTX112827
Date : Anonymous submitted on 14-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 5 μL
Blocking : 3% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-GADD153 antibody [N1C3] used in Western Blot (WB). GTX112827
Date : Anonymous submitted on 12-Jul-2019
Score :
Application Tested : WB
Sample Species : Rat
Sample : PC12 cells
Amount used : 40 μg
Blocking : 5% BSA
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
Application Reference
Wei-Chun Chen et al. Viruses 2021; 13 (4) (E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression.
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187:113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Rauti R et al. Arch Toxicol 2020; 94 (3):845-855 Bilirubin disrupts calcium homeostasis in neonatal hippocampal neurons: a new pathway of neurotoxicity.
Application : WB
Reactivity : Rat
Zhu R et al. Mol Cell Endocrinol 2019; 493:110470 Defective endoplasmic reticulum export causes proinsulin misfolding in pancreatic β cells.
Application : WB
Reactivity : Mouse
Li JF et al. J Cell Physiol 2019; (Epub) Regulation of P300 and HDAC1 on endoplasmic reticulum stress in isoniazid-induced HL-7702 hepatocyte injury.
Application : WB
Reactivity : Human
Lin S et al. Int J Biochem Cell Biol 2017; 87:54-68 endoplasmic reticulum-targeting photosensitizer hypericin confers chemo-sensitization towards * through inducing pro-death autophagy.
Application : WB
Reactivity : Human
Chiang MC et al. Exp Cell Res 2015; 338 (2):183-93 Rosiglitazone activation of PPARγ-dependent signaling is neuroprotective in mutant huntingtin expressing cells.
Application : WB
Reactivity : Mouse
Karam R et al. EMBO Rep 2015; 16 (5):599-609 The unfolded protein response is shaped by the NMD pathway.
Application : IHC
Reactivity : Mouse
Lin S et al. Cell Stress Chaperones 2014; Development of a multifunctional luciferase reporters system for assessing endoplasmic reticulum-targeting photosensitive compounds.
Application : IHC-P
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169