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GALNT7 antibody [N1C2]

Orthogonal
Anti-GALNT7 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106068
Anti-GALNT7 antibody [N1C2] used in Western Blot (WB). GTX106068
Anti-GALNT7 antibody [N1C2] used in Western Blot (WB). GTX106068
Anti-GALNT7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106068
Anti-GALNT7 antibody [N1C2] used in Western Blot (WB). GTX106068

Cat. No. GTX106068

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GALNT7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

Mouse brain , A431

Predict Reactivity

Zebrafish, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GALNT7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950335

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polypeptide N-acetylgalactosaminyltransferase 7 , GALNAC-T7 , GalNAcT7

Cellular Localization

Golgi apparatus membrane; Single-pass type II membrane protein

Background

This gene encodes GalNAc transferase 7, a member of the GalNAc-transferase family. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GALNT7 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106068

GTX106068 ICC/IF Image

GALNT7 antibody [N1C2] detects GALNT7 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALNT7 protein stained by GALNT7 antibody [N1C2] (GTX106068) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-GALNT7 antibody [N1C2] used in Western Blot (WB). GTX106068

GTX106068 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX106068 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNT7 antibody [N1C2] used in Western Blot (WB). GTX106068

GTX106068 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX106068 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GALNT7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106068

GTX106068 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endometrial cancer, using GALNT7(GTX106068) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GALNT7 antibody [N1C2] used in Western Blot (WB). GTX106068

GTX106068 WB Image

Orthogonal

The data was published in the journal PLoS One in 2016. PMID: 27281159

REFERENCE

Application Reference

Nagai-Okatani C et al. PLoS One 2016; 11 (6) : e0150210 Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169