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GBE1 antibody [1D11]

Overexpression
Anti-GBE1 antibody [1D11] used in Western Blot (WB). GTX84452
Anti-GBE1 antibody [1D11] used in Flow cytometry (FACS). GTX84452
Anti-GBE1 antibody [1D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84452
Anti-GBE1 antibody [1D11] used in IHC (Paraffin sections) (IHC-P). GTX84452
Anti-GBE1 antibody [1D11] used in Western Blot (WB). GTX84452
Anti-GBE1 antibody [1D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84452
Anti-GBE1 antibody [1D11] used in Flow cytometry (FACS). GTX84452
Anti-GBE1 antibody [1D11] used in IHC (Paraffin sections) (IHC-P). GTX84452
Anti-GBE1 antibody [1D11] used in IHC (Paraffin sections) (IHC-P). GTX84452
Anti-GBE1 antibody [1D11] used in Immunoprecipitation (IP). GTX84452
Anti-GBE1 antibody [1D11] used in Western Blot (WB). GTX84452

Cat. No. GTX84452

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse
Package
100 μl ($519)
See all GBE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:50-1:100
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

1,4-alpha-glucan branching enzyme 1 , APBD , GBE , GSD4

Background

Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells.

Database

Research Area

DATA IMAGES

Anti-GBE1 antibody [1D11] used in Western Blot (WB). GTX84452

GTX84452 WB Image

WB analysis of various cell lines using GTX84452 GBE1 antibody [1D11].
Loading : 35 ug per lane

Anti-GBE1 antibody [1D11] used in Flow cytometry (FACS). GTX84452

GTX84452 FACS Image

FACS analysis of Jurkat cells using GTX84452 GBE1 antibody [1D11].
Red : Primary antibody
Blue : Negative control antibody

Anti-GBE1 antibody [1D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84452

GTX84452 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with GBE1 plasmid using GTX84452 GBE1 antibody [1D11].

Anti-GBE1 antibody [1D11] used in IHC (Paraffin sections) (IHC-P). GTX84452

GTX84452 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84452 GBE1 antibody [1D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GBE1 antibody [1D11] used in Western Blot (WB). GTX84452

GTX84452 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84452 GBE1 antibody [1D11].
Loading : 10 ug per lane
Dilution : 1:500

Anti-GBE1 antibody [1D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84452

GTX84452 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84452 GBE1 antibody [1D11].

Anti-GBE1 antibody [1D11] used in Flow cytometry (FACS). GTX84452

GTX84452 FACS Image

FACS analysis of HEK293T cells transfected with either GBE1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84452 GBE1 antibody [1D11].

Anti-GBE1 antibody [1D11] used in IHC (Paraffin sections) (IHC-P). GTX84452

GTX84452 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84452 GBE1 antibody [1D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GBE1 antibody [1D11] used in IHC (Paraffin sections) (IHC-P). GTX84452

GTX84452 IHC-P Image

IHC-P analysis of human colon tissue using GTX84452 GBE1 antibody [1D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GBE1 antibody [1D11] used in Immunoprecipitation (IP). GTX84452

GTX84452 IP Image

IP analysis of DDDDK tagged GBE1 overexpressed HEK293T lysate using GTX84452 GBE1 antibody [1D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-GBE1 antibody [1D11] used in Western Blot (WB). GTX84452

GTX84452 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GBE1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84452 GBE1 antibody [1D11].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519