GBE1 antibody [4D9]

Cat. No. GTX84453

Host	Mouse
Clonality	Monoclonal
Clone Name	4D9
Isotype	IgG2a
Application	WB, ICC/IF, FACS, IP
Reactivity	Human
Package	100 μl ($519)

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Datasheet
Document

View product citations for antibody GTX84453 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
FACS	1:100
IP	2ug/500ul

Not tested in other applications.

Calculated MW

80 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

1,4-alpha-glucan branching enzyme 1 , APBD , GBE , GSD4

Background

Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells.

Database

Gene ID: 2632 GBE1
UniProt: Q04446 GBE1

Research Area

DATA IMAGES

GTX84453 IP Image

IP analysis of DDDDK tagged GBE1 overexpressed HEK293T lysate using GTX84453 GBE1 antibody [4D9].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

GTX84453 FACS Image

FACS analysis of HeLa cells using GTX84453 GBE1 antibody [4D9].
Red : Primary antibody
Blue : Negative control antibody

GTX84453 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with GBE1 plasmid using GTX84453 GBE1 antibody [4D9].

GTX84453 WB Image

WB analysis of HEK293T cells transfected with GBE1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84453 GBE1 antibody [4D9].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty