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GBP5 antibody [N1N3]

Orthogonal
Anti-GBP5 antibody [N1N3] used in Western Blot (WB). GTX106994
Anti-GBP5 antibody [N1N3] used in Western Blot (WB). GTX106994

Cat. No. GTX106994

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA-Ag

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GBP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ELISA-Ag Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GBP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950349

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

guanylate binding protein 5 , GBP-5

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Database

Research Area

DATA IMAGES

Anti-GBP5 antibody [N1N3] used in Western Blot (WB). GTX106994

GTX106994 WB Image

Orthogonal

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GBP5 antibody [N1N3] (GTX106994) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GBP5 antibody [N1N3] used in Western Blot (WB). GTX106994

GTX106994 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GBP5 antibody [N1N3] (GTX106994) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Sreevalsan S et al. EMBO Rep 2020; 21 (12) : e50155 MLLT6 maintains PD-L1 expression and mediates tumor immune resistance.
Fukumoto M et al. Cancer Sci 2014; 105 (10) : 1351-9 Guanine nucleotide-binding protein 1 is one of the key molecules contributing to cancer cell radioresistance.
Application : ELISA-Ag
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169