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GCLC antibody

Anti-GCLC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66057
Anti-GCLC antibody used in Western Blot (WB). GTX66057

Cat. No. GTX66057

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all GCLC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:3000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-252 of human GCLC (NP_001489.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-cysteine ligase catalytic subunit , GCL , GCS , GLCL , GLCLC

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-GCLC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66057

GTX66057 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66057 GCLC antibody.
Blue : DAPI
Dilution : 1:100

Anti-GCLC antibody used in Western Blot (WB). GTX66057

GTX66057 WB Image

WB analysis of various sample lysates using GTX66057 GCLC antibody.
Dilution : 1:3000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399