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GCLM antibody [AT2D12]

Orthogonal
Anti-GCLM antibody [AT2D12] used in Western Blot (WB). GTX53719
Anti-GCLM antibody [AT2D12] used in Western Blot (WB). GTX53719
Anti-GCLM antibody [AT2D12] used in Western Blot (WB). GTX53719
Anti-GCLM antibody [AT2D12] used in Western Blot (WB). GTX53719

Cat. No. GTX53719

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT2D12

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all GCLM products

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:3000.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GCLM (1-274aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887821

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-cysteine ligase modifier subunit , GLCLR

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Database

Research Area

DATA IMAGES

Anti-GCLM antibody [AT2D12] used in Western Blot (WB). GTX53719

GTX53719 WB Image

WB analysis of HeLa lysate (40ug) using GCLM antibody at a dilution of 1:3,000.

Anti-GCLM antibody [AT2D12] used in Western Blot (WB). GTX53719

GTX53719 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

Anti-GCLM antibody [AT2D12] used in Western Blot (WB). GTX53719

GTX53719 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

Anti-GCLM antibody [AT2D12] used in Western Blot (WB). GTX53719

GTX53719 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

REFERENCE

Application Reference

Bai X et al. Oxid Med Cell Longev 2019; 2019 : 2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
Application : WB
Reactivity : Human
Jeongsu Han et al. Oxidative Medicine and Cellular Longevity 2019; 2019 : Article ID 2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399