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GCLM antibody

Anti-GCLM antibody used in Western Blot (WB). GTX32622
Anti-GCLM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32622

Cat. No. GTX32622

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all GCLM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human GCLM (NP_002052.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-cysteine ligase modifier subunit , GLCLR

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Database

Research Area

DATA IMAGES

Anti-GCLM antibody used in Western Blot (WB). GTX32622

GTX32622 WB Image

WB analysis of various sample lysates using GTX32622 GCLM antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GCLM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32622

GTX32622 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32622 GCLM antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399