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GDF5 antibody

KO/KD Orthogonal
Anti-GDF5 antibody used in Western Blot (WB). GTX113580
Anti-GDF5 antibody used in Western Blot (WB). GTX113580
Anti-GDF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113580
Anti-GDF5 antibody used in Western Blot (WB). GTX113580
Anti-GDF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113580
Anti-GDF5 antibody used in Functional Assay (Functional Assay). GTX113580

Cat. No. GTX113580

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, Functional Assay

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GDF5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Mouse, Rabbit, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GDF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167042

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

growth differentiation factor 5 , BDA1C , BMP-14 , BMP14 , CDMP1 , LAP-4 , LAP4 , OS5 , SYM1B , SYNS2

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GDF5 antibody used in Western Blot (WB). GTX113580

GTX113580 WB Image

Orthogonal

Various whole cell extracts (30 Âμg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GDF5 antibody (GTX113580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-GDF5 antibody used in Western Blot (WB). GTX113580

GTX113580 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX113580 diluted at 1:1000

Anti-GDF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113580

GTX113580 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GDF5(GTX113580) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GDF5 antibody used in Western Blot (WB). GTX113580

GTX113580 WB Image

KO/KD

The data was published in the journal PLoS One in 2012. PMID: 23226264

Anti-GDF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113580

GTX113580 ICC/IF Image

The data was published in the journal Stem Cells Int in 2013. PMID: 24454406

Anti-GDF5 antibody used in Functional Assay (Functional Assay). GTX113580

GTX113580 Functional Assay Image

The data was published in the journal PLoS One in 2012. PMID: 23226264

REFERENCE

Application Reference

Bucher C et al. Stem Cells Int 2013; 2013 : 326828 Nonviral Gene Delivery of Growth and Differentiation Factor 5 to Human Mesenchymal Stem Cells Injected into a 3D Bovine Intervertebral Disc Organ Culture System.
Chen YJ et al. Life Sci 2013; 2013 : 326828 Nonviral Gene Delivery of Growth and Differentiation Factor 5 to Human Mesenchymal Stem Cells Injected into a 3D Bovine Intervertebral Disc Organ Culture System
Application : ICC/IF
Reactivity : Human
Margheri F et al. PLoS One 2012; 7 (11) : e50342 GDF5 regulates TGF--dependent angiogenesis in breast carcinoma MCF-7 cells: in vitro and in vivo control by anti-TGF peptides.
Application : Functional Assay
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169