GeneTex

  • Country / Location Selection

United States (US)

GDNF antibody

Anti-GDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX02540
Anti-GDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX02540
Anti-GDNF antibody used in Western Blot (WB). GTX02540
Anti-GDNF antibody used in Western Blot (WB). GTX02540

Cat. No. GTX02540

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine
Package
100 μl ($399)
See all GDNF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:300-1:1000
IHC-P 1:200-1:400
FACS Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Aqueous buffered solution, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived from human GDNF (166-211/211)

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial cell derived neurotrophic factor , ATF , ATF1 , ATF2 , HFB1-GDNF , HSCR3

Cellular Localization

Secreted

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-GDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX02540

GTX02540 IHC-P Image

IHC-P analysis of rat brain tissue using GTX02540 GDNF antibody.
Dilution : 1:200

Anti-GDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX02540

GTX02540 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX02540 GDNF antibody.
Antigen retrieval : Boiling in sodium citrate buffer (pH6) for 15min
Dilution : 1:400

Anti-GDNF antibody used in Western Blot (WB). GTX02540

GTX02540 WB Image

WB analysis of U251 cell (lane 1) and mouse brain tissue (lane 2) lysates using GTX02540 GDNF antibody.
Dilution : 1:300

Anti-GDNF antibody used in Western Blot (WB). GTX02540

GTX02540 WB Image

WB analysis of rat brain (lane 1) and rat heart (lane 2) tissue lysates using GTX02540 GDNF antibody.
Dilution : 1:200

REFERENCE

Application Reference

Tsai CW et al. Neurotherapeutics 2022; 19 (2) : 649-659 Transcranial Ultrasound Stimulation Reverses Behavior Changes and the Expression of Calcium-Binding Protein in a Rodent Model of Schizophrenia.
Submit a Reference

REVIEW

There are currently no reviews for GDNF antibody (GTX02540). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399