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GDNF antibody

Anti-GDNF antibody used in IHC (Frozen sections) (IHC-Fr). GTX03708
Anti-GDNF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03708
Anti-GDNF antibody used in IHC (Frozen sections) (IHC-Fr). GTX03708
Anti-GDNF antibody used in Western Blot (WB). GTX03708

Cat. No. GTX03708

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μl ($679)
See all GDNF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:200-1:500
IHC-Fr 1:100
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor) (Accession P39905), C-terminus of the mature GDNF.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial cell derived neurotrophic factor , ATF , ATF1 , ATF2 , HFB1-GDNF , HSCR3

Cellular Localization

Secreted

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-GDNF antibody used in IHC (Frozen sections) (IHC-Fr). GTX03708

GTX03708 IHC-Fr Image

IHC-Fr analysis of rat hippocampus tissue using GTX03708 GDNF antibody. GDNF staining is apparent in the pyramidal layer (asterik), in astrocytic fibers (horizontal arrows) and in some interneurons (vertical arrow).
Red : GDNF
Green : Calbindin D28k
Blue : DAPI is used as the counterstain
Dilution : 1:100

Anti-GDNF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03708

GTX03708 ICC/IF Image

ICC/IF analysis of PFA-fixed rat C6 glioma cells using GTX03708 GDNF antibody.
Red : Primary antibody
Blue : Hoechst 33342
Dilution : 1:200

Anti-GDNF antibody used in IHC (Frozen sections) (IHC-Fr). GTX03708

GTX03708 IHC-Fr Image

IHC-Fr analysis of rat dorsal root ganglion (DRG) tissue using GTX03708 GDNF antibody. GDNF staining is specific for neurons. Note that Schwann cells as well as satellite glial cells are both negative.
Dilution : 1:50
Red : Primary antibody
Blue : Hoechst 33342

Anti-GDNF antibody used in Western Blot (WB). GTX03708

GTX03708 WB Image

WB analysis of serially diluted recombinant human GDNF protein lysates using GTX03708 GDNF antibody.
Lane 1-3 : Primary antibody
Lane 4-5 : Primary antibody preincubated with GDNF blocking peptide
Dilution : 1:1000

REFERENCE

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Package List Price ($)
$ 679