APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat-2
Predict Reactivity
Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888105
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho/Rac guanine nucleotide exchange factor 2 , GEF , GEF-H1 , GEFH1 , LFP40 , NEDMHM , P40
Cellular Localization
Cytoplasm , Cell junction , tight junction , Golgi apparatus , Spindle
Background
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX629399 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629399 IHC-P Image
GEF-H1 antibody [GT1132] detects GEF-H1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GEF-H1 stained by GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629399 IHC-P Image
GEF-H1 antibody [GT1132] detects GEF-H1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.GEF-H1 stained by GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629399 WB Image
GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat-2 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000
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GTX629399 WB Image
GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000
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GTX629399 WB Image
GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extract E. 30 μg BCL-1 whole cell lysate/extract F. 30 μg Raw264.7 whole cell lysate/extract G. 30 μg C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000
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REFERENCE
There are currently no references for GEF-H1 antibody [GT1132] (GTX629399). Be the first to share your publications with this product.
REVIEW
GEF-H1 antibody [GT1132]
Cat. No. GTX629399
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 12-Jun-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
3A6 cells
Amount used :
30μg
Blocking :
3% BSA in TBST, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 16Hr