APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 5-10μg/ml |
|
Note :
IHC-P
Human Kidney shows distinct staining in the cytoplasm of epthelial cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88337-PEP).
Product Note
This antibody is expected to recognize both isoforms (NP_000812.2; NP_001135741.1).
Predict Reactivity
Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-PPESNPDPVHSE, from the C Terminus of the protein sequence according to NP_000812.2; NP_001135741.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
gamma-glutamyl carboxylase , VKCFD1
Cellular Localization
Endoplasmic reticulum membrane
Background
This gene encodes an integral membrane protein of the rough endoplasmic reticulum that carboxylates glutamate residues of vitamin K-dependent proteins to gamma carboxyl glutamate, a modification that is required for their activity. The vitamin K-dependent protein substrates have a propeptide that binds the enzyme, with carbon dioxide, dioxide, and reduced vitamin K acting as co-substrates. Vitamin K-dependent proteins affect a number of physiologic processes including blood coagulation, prevention of vascular calcification, and inflammation. Allelic variants of this gene have been associated with pseudoxanthoma elasticum-like disorder with associated multiple coagulation factor deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
Database
Research Area
DATA IMAGES
|
GTX88337 IHC-P Image
IHC-P analysis of human kidney using GTX88337 GGCX antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
|
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GTX88337 WB Image
WB analysis of human muscle lysate using GTX88337 GGCX antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for GGCX antibody, C-term (GTX88337). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GGCX antibody, C-term (GTX88337). Be the first to share your experience with this product.
