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GIRK2 antibody

Anti-GIRK2 antibody used in Western Blot (WB). GTX54832
Anti-GIRK2 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX54832
Anti-GIRK2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54832

Cat. No. GTX54832

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IP, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all GIRK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Golden Syrian Hamster(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

GST fusion protein with sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG, corresponding to residues 374-414 (Intracellular, C-terminal part) of mouse Kir3.2 (Accession P48542), (MW: 31 kDa.).

Purification

Depleted of anti-GST antibodies by affinity chromatography on immobilized GST and purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

potassium inwardly-rectifying channel, subfamily J, member 6 , BIR1 , GIRK2 , KATP2 , KCNJ7 , Kir3.2 , weaver , wv

Cellular Localization

Membrane; Multi-pass membrane protein

Database

Research Area

DATA IMAGES

Anti-GIRK2 antibody used in Western Blot (WB). GTX54832

GTX54832 WB Image

IHC-Frfl (free floating) analysis of rat substantia nigra tissue using GTX54832 GIRK2 antibody. Cell nuclei are stained with DAPI (blue).
Panel A : Kir3.2 (red) appears in cells of the substantia nigra pars compacta (SNC, arrows).
Panel B : KCNK2 (green) appears in both compacta (SNC) and reticulata (SNR) portions of the substantia nigra.
Panel C : Merge of the two images reveals co-localization in some cells (arrows), mainly in the SNC region.
Dilution : 1:120

Anti-GIRK2 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX54832

GTX54832 IHC (Free Floating) Image

IHC-Fr analysis of mouse brain tissue using GTX54832 GIRK2 antibody. GIRK2 staining (red) appears in cells and processes along the pars compacta of the mouse substantia nigra (horizontal arrows) and in the pars reticulata (vertical arrows). Cell nuclei are stained with DAPI (blue).
Dilution : 1:400

Anti-GIRK2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54832

GTX54832 IHC-Fr Image

WB analysis of rat brain membrane lysate using GTX54832 GIRK2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639