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GLI3 antibody

Anti-GLI3 antibody used in Western Blot (WB). GTX104362
Anti-GLI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104362
Anti-GLI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104362
Anti-GLI3 antibody used in Western Blot (WB). GTX104362
Anti-GLI3 antibody used in Western Blot (WB). GTX104362
Anti-GLI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104362
Anti-GLI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104362

Cat. No. GTX104362

Reference 6
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GLI3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

170 kDa. ( Note )

Positive Control

Jurkat , mouse ESC , PC-12 , Rat2

Predict Reactivity

Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLI3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171955

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GLI family zinc finger 3 , ACLS , GCPS , GLI3-190 , GLI3FL , PAP-A , PAPA , PAPA1 , PAPB , PHS , PPDIV

Cellular Localization

Nucleus

Background

This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GLI3 antibody used in Western Blot (WB). GTX104362

GTX104362 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
5% SDS PAGE
GTX104362 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104362

GTX104362 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3(GTX104362) antibody at 1:500 dilution.

Anti-GLI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104362

GTX104362 IHC-P Image

GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
GLI3 antibody (GTX104362) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GLI3 antibody used in Western Blot (WB). GTX104362

GTX104362 WB Image

Sample (20 μg of whole cell lysate)
A: mouse ESC
5% SDS PAGE
GTX104362 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLI3 antibody used in Western Blot (WB). GTX104362

GTX104362 WB Image

GLI3 antibody detects GLI3 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
GLI3 antibody (GTX104362) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104362

GTX104362 IHC-P Image

The data was published in the journal Elife in 2016. PMID: 27185526

Anti-GLI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104362

GTX104362 IHC-P Image

The data was published in the journal Cell Commun Signal in 2014. PMID: 24548465

REFERENCE

Application Reference

Kurtovi? M et al. Cancers (Basel) 2022; 14 (18) RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines.
Application : WB
Reactivity : Human
Diana Trnski et al. Sci Rep 2021; 11 (1) : 14880 SHH-N non-canonically sustains androgen receptor activity in androgen-independent prostate cancer cells.
Application : WB
Reactivity : Human
Zub?i? V et al. Int J Mol Sci 2020; 21 (17) GANT61 and Lithium Chloride Inhibit the Growth of Head and Neck Cancer Cell Lines Through the Regulation of GLI3 Processing by GSK3β.
Sato Y et al. Am J Pathol 2018; (Epub) Blockade of Hedgehog Signaling Attenuates Biliary Cystogenesis in the Polycystic Kidney (PCK) Rat.
Application : ICC/IF
Reactivity : Rat
Matz-Soja M et al. Elife 2016; 5 Hedgehog signaling is a potent regulator of liver lipid metabolism and reveals a GLI-code associated with steatosis.
Application : WB, IHC-P
Reactivity : Mouse
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REVIEW

GLI3 antibody Cat. No. GTX104362

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-GLI3 antibody used in Western Blot (WB). GTX104362
Date : Anonymous submitted on 27-Jan-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : LNCaP cells
Blocking : 5% milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production